Journal: Journal of Nutrition and Metabolism
Article Title: Saturated Fatty Acid-Induced Cytotoxicity in Liver Cells Does Not Involve Phosphatase and Tensin Homologue Deleted on Chromosome 10
doi: 10.1155/2013/514206
Figure Lengend Snippet: Cell viability in H4IIE and HepG2 liver cells. ELISA-based cell death (a), MTT assay (b), and caspase-3 activity (c) in H4IIE (left column) and HepG2 (right column) liver cells following 16 h of incubations with control media (LG) or fatty acids (oleate (O), linoleate (L), palmitate (P), or stearate (S)) at 250 μ M in the absence (Control) or presence of PTEN knockdown (SiRNA) ( n = 6). *: significantly ( P < 0.05) different from LG, O250, and L250.
Article Snippet: The rat hepatoma liver cell line H4IIE and the human hepatoma cell line HepG2 (American Type Culture Collection, Manassas, VA, USA) were cultured in DMEM containing 8 mM glucose and supplemented with 10% fetal bovine serum, penicillin, and streptomycin sulfate.
Techniques: Enzyme-linked Immunosorbent Assay, MTT Assay, Activity Assay, Control, Knockdown